vahidVahid mohammadi; seyed davood sharifi; Mohsen Sharafi; abdollah mohammadi sang cheshmeh
Volume 22, Issue 2 , June 2020, , Pages 271-279
Abstract
The effect of feeding L-carnitine during pre-puberty on the quality parameters of fresh and frozen-thawed semen by using 12 Ross broiler breeder males (12 weeks) for 18 weeks, in a completely randomized design with three treatments (0, 250 and 500 mg / kg of L-carnitine in the diet) and four replications ...
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The effect of feeding L-carnitine during pre-puberty on the quality parameters of fresh and frozen-thawed semen by using 12 Ross broiler breeder males (12 weeks) for 18 weeks, in a completely randomized design with three treatments (0, 250 and 500 mg / kg of L-carnitine in the diet) and four replications was performed. From the age of 26 to 29 weeks, semen collection was performed using abdominal massage. The sperms taken each time after dilution (with Beltsville diluent) were divided into two parts, one section was frozen and the other part was immediately examined. Motility (total and forward), viability, morphology, membrane functionality and lipid peroxidation parameters were evaluated. In fresh sperm, the correlation between L-carnitine and abnormalities was negative linear, and with viability was positive linear (P<0.05). Quadratic analysis was significant in forward Motility and MDA concentration (P<0.05). Birds that use diets containing L-carnitine, In terms of forward motility, viability, morphology and MDA concentrations in fresh sperm, And these traits, with the total motility and integrity of the plasma membrane of frozen sperm, were higher in comparison to the control group (P<0.05). Also, in the sperm after frozen-thawed, the correlation between L-carnitine and Motility (total and forward), viability and membrane integrity were positive linear (P<0.05), and the correlation between L-carnitine and MDA concentration was negative linear (P<0.05). The correlation between L-carnitine and Motility (total and forward), membrane integrity and MDA concentration were quadratic (P<0.05). According to the results, Dietary L-carnitine supplementation in pre-puberty improves the qualitative traits of sperm before and after freezing in the breeder broilers.
hossein pasha zanussi; farid shariatmadari; Mohsen sharafi; Hamed Ahmadi
Volume 22, Issue 1 , March 2020, , Pages 79-92
Abstract
This study was conducted to assess the effects of dietary flaxseed and sesame oils, on the semen parameters, fatty acid composition of sperm as well as the fertility and hatchability eggs from aged roosters. In a completely randomized design, 24 Ross-308 roosters (aged 45 week) assigned to ...
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This study was conducted to assess the effects of dietary flaxseed and sesame oils, on the semen parameters, fatty acid composition of sperm as well as the fertility and hatchability eggs from aged roosters. In a completely randomized design, 24 Ross-308 roosters (aged 45 week) assigned to four groups, comprising six replicates and one bird in each. The birds received different diets including basal diet (control), basal diet supplemented with 2% flaxseed oil (FO), basal diet supplemented with 2% sesame oil (SO) and basal diet supplemented with 1% flaxseed oil and 1% sesame oil (MO). The diets were iso-caloric and iso-nitrogenous, containing the same level of vitamin E. The roosters were fed diet for 60 days, during which semen samples were collected on 1st, 20th, 40th and 60th days and the samples were tested for different characteristics. The results indicated that different diets affected semen qualities, except semen volume and the morphology. The concentration, progressive motility, MDA as well as viability of sperms were significantly different during different times of the experiment. The sperm quality parameters including total and progressive motility as well as MDA turned out to improve in the roosters fed FO or MO. Furthermore, the integrity of sperm membrane, DHA and DPA concentration, as well as the fertility were higher in the treatment group containing FO. It seems that supplementation of aged rooster’s diet with flaxseed oil or mixed oils, together with vitamin E improves the semen qualities and it can be applied as an appropriate strategy to preserve the reproductive performance of aged rooters.
Mostafa Lotfy; farid shariatmadari; Hamed Ahmadi; Mohsen Sharafi
Volume 21, Issue 2 , July 2019, , Pages 223-232
Abstract
The purpose of this study was to develop multiple linear regression (MLR) model to predict the nitrogen-corrected true metabolizable energy (TMEn) value of wheat bran. The amount of crude fat, ash, crude protein, crude fiber (all used as % of DM) and TMEn (Kcal/kg DM) were measured in 25 wheat bran samples ...
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The purpose of this study was to develop multiple linear regression (MLR) model to predict the nitrogen-corrected true metabolizable energy (TMEn) value of wheat bran. The amount of crude fat, ash, crude protein, crude fiber (all used as % of DM) and TMEn (Kcal/kg DM) were measured in 25 wheat bran samples with 4 replicates. The force-fed method has been used to estimate TMEn and excreta were collected for 48 h. There were significant (P < 0.001) differences in chemical composition and TMEn of wheat bran samples. The average crude fat, ash, crude protein, crude fiber and TMEn content of samples was determined to be 4.80, 5.68, 16.23, 8.60 (all used as % of DM) and 2062 (Kcal/kg DM), respectively. The calculated MLR model to predict the TMEn value (Kcal/kg) based on chemical composition (% of DM) was obtained as follows: TMEn = 2364 + (19×crude protein) + (46.1×crude fat) – (63×crude fiber) – (51.1×ash). The R2 value revealed that developed model could accurately predict the TMEn of wheat bran samples (R2=0.82). Crude fat and crude protein had a positive effect on TMEn, while ash and crude fiber had a negative impact on TMEn. The sensitivity analysis on the model indicated that dietary crude fiber (%) is the most important variable in the TMEn, followed by dietary ash, crude fat and crude protein. The results suggest that the MLR model may be used to accurately estimate the TMEn value of wheat bran from its corresponding chemical composition.
Mehdi Noorani; Shaban Rahimi; Abdolhoseein Shahverdi; Mohsen Sharafi
Volume 20, Issue 2 , August 2018, , Pages 203-212
Abstract
The aim of this study was cloning of the beta subunit of human chorionic gonadotropin in an appropriate vector for production of transgenic chicken trrough sperm mediated gene transfer. In this regard, transgenic chicken production tecnology has taken into consideration for having many advantages such ...
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The aim of this study was cloning of the beta subunit of human chorionic gonadotropin in an appropriate vector for production of transgenic chicken trrough sperm mediated gene transfer. In this regard, transgenic chicken production tecnology has taken into consideration for having many advantages such as short generation times, the large number of production of offspring and suitable pattern of protein glycosylation. To date, no study has been conducted on the cloning of the beta subunit of human chorionic gonadotropin for rooster sperm. For this purpose, the hormone beta subunit were amplified by a specific primer pairs, and cloned in T vector. The recombinant plasmid was transformed into Competent E. coli cells and colonies that containing recombinant plasmids were selected by colony PCR.The validity of extracted plasmid were analyzed by enzyme digestion and sequencing. The beta chain of T vector was isolated and was cloned again into pcDNA3.1 + expression vector. The results of enzyme analysis and sequencing indicated that recombinant plasmid pCDNA3.1 +/βhCG were cloned with the correct sequence and completely matched up with human chorionic gonadotropin beta subunit gene that can be concluded that it has sutible stracture for sperm mediated gene transfer.
farid shariatmadari
Volume 19, Issue 2 , August 2017, , Pages 479-491
Abstract
In this study, the effect of fat source and type of growth promoting additives in laying hens diets on productive performance and egg quality traits was investigated. In a (2×3) factorial experiment,108 ninety-six week-old Hy-Line W-36 laying hens treated with 2 fat sources (vegtable fat - yellow ...
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In this study, the effect of fat source and type of growth promoting additives in laying hens diets on productive performance and egg quality traits was investigated. In a (2×3) factorial experiment,108 ninety-six week-old Hy-Line W-36 laying hens treated with 2 fat sources (vegtable fat - yellow grease and fat powder) and 3 types of growth promoting additives (none-additive,probiotic and antibiotic) for 12 weeks.The birds were allocated randomly in six treatments with six replicates (3 birds per replicat). Results showed that None of the performance variables (egg production rate, egg mass, average egg weight, feed intake and feed conversion ratio) were affected by the supplemental fat sources (P> 0.05). The addition of probiotic in feed resulted in significant reductions in feed intake, egg production and egg mass as well as in yolk percent (P< 0.05). Interactions between fat source and growth promoting additive type as well as the main effect of fat type on Egg shell thickness were significant. Thus, it could be suggested that the use of the fat sources and growth promoting additives in layer diets as a potential solution to prevent the egg shell quality problems during the late stages of production; however supplemental probiotic reduced feed intake, egg production and egg mass.
behrooz shokrani; morteza mehri; amir fattah; mohsen sharafi; fatemeh shirmohammad
Volume 18, Issue 4 , December 2016, , Pages 853-865
Abstract
Effects of dietary rosemary (Rosmarinus officinalis) essential oil were evaluated on the semen quality of broiler breeder rooster using sixteen 24-wk Ross 308 male birds. Experimental groups were consisting of four treatments (0, 50, 100 and 200 mg rosemary essential oil/kg of feed) with four replicates ...
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Effects of dietary rosemary (Rosmarinus officinalis) essential oil were evaluated on the semen quality of broiler breeder rooster using sixteen 24-wk Ross 308 male birds. Experimental groups were consisting of four treatments (0, 50, 100 and 200 mg rosemary essential oil/kg of feed) with four replicates per each group. Semen samples were collected on days 0, 14, 28, 42 and 56 of experiment. Results showed that 100mg rosemary significantly improved several traits of sperm such as Amplitude of Lateral Head Displacement (ALH), Average Path Velocity (VAP), and Straight Line Velocity (VSL) on days 42 and 56, and sperm Membrane Integrity (MI), sperm viability, Linearity (LIN) and Total Motility (TM) on day 56 (P<0.05) and significantly decreased dead sperms (P<0.05). However, sperm concentration, apoptosis and morphological changes, Sperm Track Straightness (STR), Curvilinear Velocity (VCL), Progressive motility (PM) and malondialdehyde (MDA) level were not affected by experimental treatment. It can be concluded that 100 mg rosemary essential oil/kg of diet could be efficient for improvement of some qualitative sperm parameters.